Systematization of the offering of a diet for inducing obesity in adult rats
Abstract
Aim: To systematize the offering of foods which were highly palatable and rich in calories and to investigate the effects on morphometric and metabolic parameters of adult Wistar rats. Methods: The study used 15 male Wistar rats, aged 45 days old, divided into a control group and a cafeteria diet group. To the control group was offered a commercial diet ad libitum, while to the cafeteria diet group was offered the commercial diet supplemented each day with four items selected from a list of 28 foods. The consumption of foods from the cafeteria diet was checked daily, and the two groups' consumption of water and feed was checked weekly. After seven weeks, the morphometric parameters were checked and animals were sacrificed for collection of blood and the deposit of perigonadal fat. Results: The mean weight of the cafeteria diet group was higher than that of the control group, as was abdominal circumference, deposit of perigonadal fat, body mass index and the Lee index. Furthermore, animals from the cafeteria diet group presented high blood levels of triglycerides; also, changes were not observed in levels of cholesterol and glucose. Animals from the cafeteria diet group ingested more calories, carbohydrates and fats than animals in the control group. Conclusion: This study showed that a protocol of offering a cafeteria diet formulated based in foods typically consumed by the Brazilian population can be used for inducing obesity in rats, requiring a relatively short time period for obtaining results.
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