Phytoestrogen Secoisolaciresinol Diglicosideo changes levels the estradiol and relative body of ovariectomized female rats Wistar
Abstract
Aims: To evaluate the influence of phytoestrogen Secoisolaciresinoldiglicosídeoflaxseed over a long time period and its hormonal effects on weight loss in castrated rats. Materialsand Methods: Wistar rats 21that underwent bilateral oophorectomy for induction of menopause were randomly divided into three groups: Control group(n=7) received diet based on10% casein, Flaxseed group(n=7) received flaxseed diet based on casein plus 25% flaxseed and the Modified control group (n=7) received modified diet based on 10% casein with the addition of lipid and fiber. All diets were prepared according to the recommendations of the AIN 93, and the animals received water ad libitum. The rats were anesthetized with Thiopentax® for blood collection by cardiac puncture to determine 17β-estradiol. The acquired data were statistically analyzedby the nonparametric Kruskal-Wallis test and post hoc Mann-Whitney U for comparison two by two through Software S-Plus version 8.0, with a 0.05 level for the significant differences. Results:At the end of 180-day experimentwe observed that the results suggest that SDG is present in flaxseed at 13.5± 0.4 mg/g, feed intake in FG(1641.1±0.9), CG (1478.4±0.1) and MCG (1605.8±1.8) showed no significant difference between groups. Have the mass on animals body it is observed thatthe FG (275.5±33.9), CG (294.0±33.1) and MCG (339.5 ± 24.6), suggesting that was no statistical difference between groups (p<0.002). Serum levels of 17β-estradiol showed higher values in FG (48.7 ± 12.31 pg/dl), GC (29.0±5.4 pg/dl), MCG (36.0±5.60 pg/dl), withstatistical differences (p = 0.0012). Conclusion: The consumption of Secoisolaciresinol Diglicosídeoin food intakedoes not influenceon body mass of animals and levels of estradiol in ovariectomized rats.
References
-Arjmandi, B.H. The role of phytoestrogens in the prevention and treatment of osteoporosis in ovarian hormone deficiency. J Am Coll Nutr. Vol. 20. Núm. 5. p. 398S-402S. 2001.
-Association of Official Analytical Chemists. Official methods of analysis. AOAC. 16. Washington. 1995.
-Begum, N.A.; Nicole, C.; Milla, I.; Lapierre, C.; Nagano, F. K. Dietary lignins precursors of mammalian lignans in rats. J Nutr. Vol. 134. Núm. 1. p. 120-127. 2003.
-Bessa, T.R.G.; Boaventura, G.T.; Vellarde, L.G.C. Phytoestrongens the modulador selective receptor estrogen? Rev Cien Med e biológicas. p. 67-70. 2011.
-Bhathena, S. J.; Velasquez, M. T. Beneficial role of dietary phytoestrogens in obesity and diabetes. Am J Clin Nutr. Vol. 76. p. 1191-1201. 2000.
-Biondo-Simões, M.L.P.; Zimmermann, E.; Daher, T.S.; Borsato, K.S.; Noronha, L. Efeitos da terapia de reposição hormonal na cicatrização de anastomoses de cólon. Acta Cir Bras. Vol. 20. p. 237-240. 2005.
-Branca, F.; Lorenzetti, S. Health effects of phytoestrogens. Forun Nutr. Itália. Vol. 57. p. 100-111. 2005.
-Brooks, J.D.; Ward, W.E.; Lewis, J.E.; Hilditchi, J.; Nickell, L.; Wong, E.; Thompsom, L.U. Supplementation with flaxseed alters estrogen metabolism in postmenopausal womwn to a grater extent than does supplementation with an equal amount of soy. Am J Clin Nutr. Vol. 79. 2004.
-Cardozo, L.F.M.F.; Soares, L.L.; Chagas, M.A.; Boaventura, G.T. Consumo de semente de linhaça durante a lactação afeta peso e nível de hemoglobina na prole de ratas. Maternal consumption of flaxseed during lactation affects weight and hemoglobin level of offspring in rats. J. pediatr. (Rio J.) Vol. 86. Núm. 2. p. 126-130. 2010.
-Carreau, C.; Gilles, F.; Catherine, B.; Potier, M. Enterodiol and enterolactone, two major diet-deriveted polyphenol metabolites have different impacto on Erαtranscriptional activation in human breast câncer cells. Journal of Steroid Biochemistry & Molecular Biology. Vol. 11. p. 176-185. 2008.
-Carusi, D. Phytoestrogens as hormone replacement therapy:an evidence-based approach. Primary care update for OB/GYNS. Vol.7. Núm. 6. p. 253-259. 2009.
-Chilibeck, P.D.; Cornish, S.M. Effect of estrogen compounds (estrogenor phytoestrogens) combined with exercise on bone and muscle mass in older individuals. Appl Physiol Metab Nutr. Vol. 33. Núm. 1. p. 200-212. 2008.
-Clapauch, R.; Meirelles, R.M.R.; Julião, M.A.; Kohler, S.G.; Loureiro, C.C.; Giarodoli, P.B.; Pinheiro, S.A.; Harrigan, A.R.; Spritzer, P.M.; Pardini, D.P.; Weiss, R.V.; Athayde, A.; Russo, L.A.; Povoa, L.C. Fitoestrogênios: Posicionamento Do Departamento De Endocrinologia Feminina Da Sociedade Brasileira de Endocrinologia E Metabologia (SBEM). Arq Bras Endocrinol Metab. Vol. 46. Núm. 6. p. 679-695. 2002.
-Cordeiro, R.; Fernandes, P.L.; Barbosa, L.A. Semente de linhaça e o efeito de seus compostos sobre as células mamárias. Rev. Bras. Farmacogn. Vol.19. Núm. 3. p. 727-732. 2009.
-Dalais, F.S.; Rice, G.E.; Walhlqvist, M.L.; Grehan, M.; Murkies, A.L. Effects of dietaryphytoestrogens in postmenopause women. Climateric. Vol. 1. Núm. 2. p. 124-129. 1998.
-Dodin, S.; Lemay, A.; Jacques, H.; Légare, F.; Forest, J-C.; Massê, B. The effects of flaxseed dietary supplement on lipid profile, bone mineral density, and symptoms in menopausal women: a randomized, double-blind, wheat germ placebo-controlled clinical trial. J Clin Endocrinol Metab. Vol. 90. Núm. 3. p. 1390-1397. 2008.
-Figueiredo, M. S. Effects of maternal flaxseed diet during lactation on body composition and thyroid function in adult animals. Tese de Doutorado. UERJ. Rio de Janeiro. 2011.
-Hallund, J.; Ranv-Haren, G.; Bügel, S.; Tholstrup, T.; Tetens, I. A lignan complex isoled from flaxseed does not affect plasma lipid concentrations or antioxidant capacity in walt menopause women.J Nutr. Vol. 136. p. 112-116. 2006.
-Hu, C.; Yuan, Y.V.; Kitts, D.D. Antioxidant Activities of The Flaxseed Lignan Secoisolariciresinol Diglucoside, Its Aglycone Ecoisolariciresinol And The Mammalian Lignans Enterodiol And Enterolactone In Vitro. Food And Chem Tox. Vol. 45. p. 2219-2227. 2007.
-Hutchins, A.M.; Slavin, J.L. Effects of flaxseed on sex hormones metabolism. In: Flaxseed in Human Nutrition, eds Thompson, L.U.; Cunnane, S.C. 2ª edição. AOCS Press. Champaign. p. 126-149. 2003.
-Kuijsten, A.; Bas, H.; Bueno, M.; Jolanda, M.; Boer, I. C.; Arts, F.J.; Pieter, V.; Peter, H. Plasma enterolignans are not associated with nonfatal myocardial infarction risk. Atherosclerosis. Vol. 203. p. 145-152. 2009.
-Miller. O Laboratório para o Clínico. Editora Atheneu. 3ªEdição. p. 10-15. 1977.
-Morris, D.H.Essential nutrients and other functional compounds in flaxseed. Nutr. Today. Vol. 36. p. 159-162. 2001.
-O’Neil, M.R. Estradiol-17β and linseed meal interact to alter visceral organ mass and hormone concentrations from ovariectomized ewes. Domestic Animal Endocrinoly. Vol. 31. p. 148-158. 2009.
-Pelletier, S.; Krundat, S.; Haslet, C.M. Effect of na educational program on intent to consume functional foods. J Am. Diet. Ass. Vol. 102. Núm. 9. p. 1297-1300. 2000.
-Prasad, K. Flaxseed: A Source of Hypocholesterolemic and Antiatherogenic Agents. Drug News Perspect. Vol. 13. Núm. 2. p. 99-104. 2000.
-Puig-Duran, E.; Greenstein, B.D.; MacKinnon, P.C.B. Effects of serum o estrogen-binding components on the unbound oestradiol-17ß fraction in the serum of developing female rats and on inhibition of o estradiol uptake by uterine tissue in vitro. J. Reprod. Fert. Vol. 56. p. 707-714. 1979.
-Reeves, P.G.; Nielsen, F.H.; Fahey Junior, G.C.F. AIN-93 purified diet of laboratory rodents: final report of the American Institute of Nutrition ad hoc Writing Committee on the Reformulation of the AIN-76A rodent diet. J Nutr. Vol. 123. p. 1939-1951. 1993.
-Riediger, N.D.; Othman, R.; Fitz, E.; Pierce, G.N.; Suh, H.; Moghadasian, M.H. Mínimo n-6: n-3 ratio de ácidos graxos, com peixe ou a óleo de linhaça, em uma dieta rica em gordura aumenta lipídios plasmáticos e benéfica altera a composição de ácidos graxos de tecidos em camundongos. Eur J Nutr. Vol. 47. Núm. 3. p. 153-160. 2008.
-Ruggiero, R. Estrogen: physiology, pharmacology, and formulations for replacement therapy. J of Midwifery & Womens Health. Vol. 47. Núm. 3. 2000.
-Serock, M.R.; Wells, A.K.; Khalil, R.A. Modulator receptor sex hormones vascular andits effects Iestrogen deficiency states associated with menopause. Recent Pat Cardiovasc Drug Discov. Vol. 3. Núm. 3. p. 165-186. 2008.
-Shoppen, B.S.; Acarbajal, A.M.; Pérez-Granados, V. M.; Pilar, V. Food energy and macronutriente intake of posmenopausal women from a menopause program. Nutr. Hospitalaria. Vol. 2. p. 101-109. 2005.
-Soares, L.L.; Lucas, A.M.M.; Boaventura, G.T. Can organic and transgenic soy be used as a substitute for animal protein byrats? Braz. J. Med. Biol. Res. Vol. 38. Núm. 4. p. 583-586. 2005.
-Soares, L.L.; Pacheco, J.T.; Brito, C.M.; Troina, A.A.; Boaventura, G.T.; Guzmán-Silva, M.A. Evaluation of the effects of flaxseed when used as a protein source for the growth and maintenance phases of rats,Rev. nutr. Vol. 22. Núm. 4. p. 483-491. 2009.
-Stringheta, P.C.; Oliveira, T.T.; Gomes, R.C.; Amaral, M.P.H.; Carvalho, A.F.; Vilela, M.A.P. Políticas de saúde e Alegações de propriedades funcionais e de saúde para alimentos no Brasil. Rev Bras Cienc Farm. Vol. 43. Núm. 2. p. 181-194. 2007.
-Szabo, J.; Ibrahim, W.H.; Brown, M. Food restriction supresses muscle growth and augments osteopenia in ovariectomized rats. J. Appl Physiol. Vol. 88. p. 265-271. 2000.
-Wang, L.; Chen, J.; Thompson, L.U. Theinhibitory effect offlaxseedon the growthand metastasisof breast cancerestrogen receptornegativehumanxenograftsisattributedto bothitslignanandoilcomponent. Int J Cancer. Vol. 116. Núm. 5. p. 793-798. 2005.
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