Assessment of lipid oxidation and physicochemical composition of omega-3 supplements in gastrointestinal simulation in vitro

  • Rafaela Vitória Utteich Universidade Regional Integrada do Alto Uruguai e das Missões, Erechim, Rio Grande do Sul, Brasil.
  • Ilizandra Aparecida Fernandes Universidade Regional Integrada do Alto Uruguai e das Missões, Erechim, Rio Grande do Sul, Brasil.
  • Marcieli Peruzzolo Universidade Regional Integrada do Alto Uruguai e das Missões, Erechim, Rio Grande do Sul, Brasil.
  • Janine Martinazzo Universidade Regional Integrada do Alto Uruguai e das Missões, Erechim, Rio Grande do Sul, Brasil.
  • Clarice Steffens Universidade Regional Integrada do Alto Uruguai e das Missões, Erechim, Rio Grande do Sul, Brasil.
Keywords: Fatty acids, Oils and fats quality, Absorption

Abstract

Objective: In recent years, in vitro gastrointestinal simulation has emerged as an essential method for understanding the interaction of foods in the gastrointestinal tract. This pioneering study investigated the physicochemical composition and lipid oxidation of omega-3 oil supplements, identified as oil 1 and oil 2, purchased locally. Materials and Methods: The research, based on packaging criteria, IFOS certification, concentrations of EPA and DHA, and presence of vitamin E, focused on in vitro simulation of the gastrointestinal tract for a detailed evaluation of the behavior of these supplements. Physicochemical analyzes were carried out, including peroxide, acidity, iodine, ester, refraction and saponification indices. To evaluate in vitro digestibility, the study simulated the digestive process in representative stages of the gastrointestinal tract, analyzing oxidation through substances reactive to Thiobarbituric Acid (TBARS). Results: During the digestive system simulation, the absorption of fatty acids was more prominent in the small intestine. Discrepancies were observed between the samples in contact with the ileum, indicating greater lipid oxidation in oil 2 compared to oil 1. The descending colon stage (48h) revealed the greatest oxidation of the oils, suggesting the need for additional studies to better understand fatty acid metabolism. Conclusion: Based on the results, omega-3 digestibility varied in different phases of the simulated gastrointestinal system, with notable absorption in the small intestine and greater oxidation in the descending colon for both samples. The difference in oxidation between the oils suggests variations in the stability of the compounds.

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Published
2024-12-29
How to Cite
Utteich, R. V., Fernandes, I. A., Peruzzolo, M., Martinazzo, J., & Steffens, C. (2024). Assessment of lipid oxidation and physicochemical composition of omega-3 supplements in gastrointestinal simulation in vitro. Brazilian Journal of Obesity, Nutrition and Weight Loss, 18(116), 1078-1087. Retrieved from https://www.rbone.com.br/index.php/rbone/article/view/2541
Issue
Vol. 18 No. 116 (2024)
Section
Scientific Articles - Original

Copyright (c) 2024 Rafaela Vitória Utteich, Ilizandra Aparecida Fernandes, Marcieli Peruzzolo, Janine Martinazzo, Clarice Steffens

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